Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
WB: Human, mouse and rat brain tissue lysate; SH-SY5Y, Neuro-2a and PC-12 whole cell lysates. IHC-P: Human, mouse and rat cerebrum and pancreas tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neurons and PC-12 cells. Flow Cyt (intra): Neuro-2a cells. IP: Human brain tissue lysate.
浓度
10 µl 浓度为 0.517 mg/ml
批次浓度范围 100 µl 浓度为 0.517 - 0.531 mg/ml
40 µl 浓度为 0.517 mg/ml
应用
Ab评论
说明
ICC/IF
1/50.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.