LS-F26730 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Collagen IV in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Collagen IV as low as 1 nanograms per millilter.
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This kit is for the detection of Human Collagen IV. No significant cross-reactivity or interference between Collagen IV and analogs was observed. This claim is limited by existing techniques therefore cross-reactivity may exist with untested analogs.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
50 - 1000 ng/ml
Sensitivity
1 ng/ml
Assay Time
1.5 h
Precision
Intra-Assay: CV%<9% Inter-Assay: CV%<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.
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