LS-F5563 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Collagen III in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Collagen III as low as 18.24 picograms per milliliter.
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Collagen Type III (COL3) No significant cross-reactivity or interference between Collagen Type III (COL3) and analogs was observed.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
46.88 - 3000 pg/ml
Sensitivity
18.24 pg/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Type-III Collagen is a fibrous scleroprotein in bone, cartilage, tendon, bone marrow stroma and other connective tissue. Mutations Collagen III are associated with type III and IV Ehlers-Danlos syndrome and with aortic and arterial aneurysms.
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