Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhC1q at 5 µg/mL (100 µL/well) can bind rhPentraxin 3 with a linear range of 0.08-5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Pentraxin 3/TSG-14 protein
Glu18-Ser381, with a C-terminal 6-His tag
N-terminal Sequence
Analysis
Glu18
Structure / Form
Multimer consisting of as many as ten non-covalently and covalently linked subunits
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
41-50 kDa, reducing conditions