Anti-Profilin 1抗体[EPR6304] (ab

Key features and details

• Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
• Rabbit monoclonal [EPR6304] to Profilin 1
• Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
• Knockout validated
• Reacts with: Mouse, Rat, Human

• 描述

  兔单克隆抗体[EPR6304] to Profilin 1

• 宿主

  Rabbit

• 特异性

  The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

• 经测试应用

  适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Rat, Human

• 免疫原

  Synthetic peptide within Human Profilin 1 aa 100-200 (C terminal). The exact sequence is proprietary.

• 阳性对照

  • WB: Jurkat, JAR, HepG2 and HEK-293T cell lysates, mouse and rat brain lysate, human fetal brain lysate; IHC-P: Human colon and breast tissue; ICC/IF: HeLa, Jurkat cells; Flow Cyt (intra): HeLa cells;

• 常规说明

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  应用	Ab评论	说明
  WB	(1)	1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
  IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. Read More
  ICC/IF		1/100. For unpurified use at 1/250 - 1/500
  Flow Cyt (Intra)		1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.