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C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞

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  • 2025年12月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      复祥生物

    • 细胞类型

      普通细胞株-科研实验

    • 物种来源

      小鼠

    • 运输方式

      常温或干冰

    • 生长状态

      良好

    • 库存

      大量

    C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞

    MEM培养基(GIBCO)+10%胎牛血清。细胞货期8-10个工作日

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    CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞 的详细介绍
    ATCC® Number: CCL-226™ Price: $269.00
    Designations: C3H/10T1/2, Clone 8
    Depositors: C Heidelberger
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mus musculus (mouse)
    Morphology: fibroblast
    CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞
    Source: Strain: C3H
    Organ: embryo
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications: transfection host (Roche FuGENE® Transfection Reagents)
    Tumorigenic: No
    Reverse Transcript: negative
    Antigen Expression: H-2k
    Cytogenetic Analysis: Mouse karyotype with a modal number of 80 chromosomes.
    Age: embryo
    Comments: C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. [23019]
    The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses. [22697]
    The cells are contact sensitive.
    There is no detectable background spontaneous transformation.
    They are highly susceptible to transformation by chemical agents. [1208]
    Tested and found negative for ectromelia virus (mousepox).
    NOTE: THE INOCULATION DENSITY, FEEDING AND HARVESTING SCHEDULES MUST BE FOLLOWED RIGIDLY IF THE LINE IS TO RETAIN ITS ESSENTIAL CHARACTERISTICS.
    THE BATCH OF SERUM USED FOR GROWTH AND FOR TRANSFORMATION ASSAYS MAY AFFECT BOTH THE MORPHOLOGY OF THIS LINE AND THE RESULTS OBTAINED.
    Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks. [1208]
    The donor recommends that the line be used between the 5th and 15th passages only.
    Propagation: ATCC complete growth medium: The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C

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    • 常见细胞系细胞培养基应用选择

      细胞人骨髓性的白血病RPMI-1640, 10% 胎牛血清U937淋巴样人组织细胞淋巴瘤RPMI-1640, 10% 胎牛血清KG-1骨髓白细胞人红白血病病人骨髓IMDM, 20% 胎牛血清McCoy成纤维细胞小鼠未知MEM, 10% 胎牛血清BALB/3T3成纤维细胞小鼠胚胎DMEM, 10% 胎牛血清3T6成纤维细胞小鼠胚胎DMEM, 10% 胎牛血清A9成纤维细胞小鼠结缔组织DMEM+10% 胎牛血清+6-硫鸟嘌呤(30μg/ml)+2,6-二氨基嘌呤(30μg/ml)AtT-20上皮

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