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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 细胞类型:
科研
- 品系:
详情请咨询我司客服
- 组织来源:
详情请咨询我司客服
- 物种来源:
鼠/人/其它
- 细胞形态:
上皮样/成纤维样/其它
- 器官来源:
详情请咨询我司客服
- 运输方式:
常温运输/干冰运输
- 年限:
详情请咨询我司客服
- 生长状态:
贴壁/悬浮
- 英文名:
Tsu-Prl
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:

(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
- Examine the cell cultures after 24 hours and subculture as needed.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
非雄激素依赖型前列腺癌_Tsu-Prl相关产品:
| WISH(羊膜细胞) |
| HL-7702(肝细胞) |
| Ha Fe(羊膜细胞) |
| QSG-7701(肝细胞) |
| HFL-I(MEMα)(胚肺成纤维细胞) |
| HeLa [ Chang Liver ](张氏肝细胞) |
| V79-4(中国仓鼠肺细胞) |
| CHL/IU [ CHL-11 ](中国仓鼠肺细胞) |
| CHO(中国仓鼠卵巢细胞) |
| BHK(幼仓鼠肾细胞) |
| R 1610(中国仓鼠体细胞) |
| BRL 3A(大鼠肝细胞) |
| NRK(大鼠肾细胞) |
| L929(成纤维细胞) |
| Y1(肾上腺皮质细胞) |
| CTLL-2(T细胞) |
| BALB/3T3(BALB/C小鼠胚成纤维细胞) |
| Ana-1(巨噬细胞) |
| SRSV/3T3(SRSV转化的3T3细胞) |
| 3T6-Swiss albino(胚胎成纤维细胞) |
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文献和实验Cell Rep Med:李振斐 / 黄盛松团队确认 3βHSD1 为晚期前列腺癌治疗新靶点
主要利用药物阿比特龙(abiraterone)和恩杂鲁胺(enzalutamide)来治疗 CRPC。 阿比特龙通过抑制代谢酶 CYP17A 来减少肾上腺合成脱氢表雄酮(dehydroepiandrosterone,DHEA);恩杂鲁胺通过与雄激素竞争性结合雄激素受体(androgen receptor,AR)来延缓前列腺癌进展。当这两个药物耐受后,患者几乎无药可用。 阿比特龙和恩杂鲁胺耐受后的前列腺癌异质性增加。一方面,雄激素非依赖性的神经内分泌型前列腺癌
三句话读懂一篇 CNS:人造蛋白质药物再获突破;每天喝 2~3 杯咖啡,或降低心脏病风险
人员在美国心脏病学会第 71 届年度科学会议上报告了一项最新研究。研究通过分析英国生物样本库(UK BioBank)中超过 50 万人的数据,探索咖啡摄入量与心率问题、心血管疾病、死亡风险和心脏相关风险的相关性。 研究结果表明,每天喝 2~3 杯咖啡,不仅可以降低患心脏病的风险,还可以延长寿命。并通过分析不同咖啡类型(速溶咖啡、现磨咖啡、含咖啡因咖啡、不含咖啡因咖啡),发现无咖啡因的咖啡对预防心律失常没有积极作用。 图片来源:站酷海洛 3. Nature:雄激素受体活性影响前列腺癌疗效 前列腺癌
Immunity:邓刘福团队发现雄激素「侵蚀」T 细胞干性影响肿瘤免疫治疗
浸润性 CD8+ T 细胞的相关性,奠定了 AR 信号通路在肿瘤免疫中的重要地位。当下,AR 受体信号通路阻断剂在前列腺癌中广泛应用,该研究为相关药物在其他癌肿中的推广应用提供了坚实的理论依据,通过干预 AR 信号通路实现干细胞样 CD8+ T 细胞的重编程,成为新药开发的重要思路。 综上所述,该研究揭示了肿瘤性别差异的免疫调节新机制,阐明了干细胞样 CD8+ T 细胞分化存在性别差异,发现了雄激素参与调控肿瘤免疫逃逸的新途径,明确了内分泌系统、免疫系统和肿瘤之间的内在联系,为靶向 AR 信号通路增强肿瘤
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