In Situ Cell Death Detection K

In situ Detection of Cell Death in Articular Cartilage

with the failure to remove apoptotic cells from the tissue. The in situ detection of cell death is an important technique in studying articular cartilage as it most closely resembles the in vivo situation. The methods described here involve the use of light

Detection of apoptotic process in situ using immunocytochemical and TUNEL assays

information Moreover, as this procedure is quite complex, the use of commercial kits such as ApoTagTM (Oncor, Gaithersburg, MD, USA) and "In situ cell death detection Kit" (Boeringer-Mannheim, Germany), is recommended. Utilizing

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling

Protocol for Paraffin Sections:Dewax paraffin sections:Incubate slides, 55°C, 30 min. Xylenes, 2 times, 2 min. each 100% EtOH, 2 times, 2 min. each 95% EtOH, 2 times, 2 min. each 80% EtOH, 2 min. 75% EtOH, 2 min. 50