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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
GST-Tag Antibody
- 应用范围:
其它
- 保存条件:
低温
- 库存:
现货
- 是否单克隆:
单克隆
- 抗体英文名:
GST-Tag Antibody
- 保质期:
一年
- 抗原来源:
其它
- 宿主:
小鼠
- 供应商:
上海研卉生物科技有限公司
- 规格:
100μl
名 称:
GST-Tag 抗体
货 号:
T0007
来 源:
Mouse
应 用:
WB,ELISA
反 应:
All
浓 度:
1mg/ml
Applications:
WB: 1:3000-1:10000
Reactivity:
All
Source:
Mouse
Clonality:
Monoclonal
Clone Number:
T571
Purification:
Affinity-chromatography.
Specificity:
GST-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GST-tagged proteins.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reference Citations:
Application: WB Species:human; Sample:Not available
FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)
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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
融合表达蛋白等。比如在免疫荧光IF的实验里,同时配合EarthOx的Dylight 549荧光二抗,使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置(如图红色显示部分)。另外,做融合表达分析前,通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步,因此选择一个合适的、可用于免疫共沉淀(Immunoprecipitation,IP)Flag标签抗体也是很必要的。EarthOx的Anti-Flag Tag Monoclonal Antibody(货号#E022060)不仅能应用
应用。用GST融合表达系统表达外源基因时,需要对融合表达的产物进行检测和纯化,这里面就包括了GST标签抗体的应用,如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源,比如小鼠源的单克隆抗体还是兔源的多克隆抗体,一般来说,单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6](货号#E022040)是来源于小鼠的单克隆抗体,克隆号为GST.B6,免疫原为GST多肽 。
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