细胞培养 : (1)Getting Started with an ATCC Cell Line: ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as their viability will decline rapidly.
(2)细胞图片:
(3)Initiating Frozen Cultures: 1. Prepare a culture vessel so that it contains the recommended volume of the appropriate culture medium as listed on the Product Sheet, equilibrated for temperature and pH (CO₂). 2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted. 3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol. Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations. 4. Unscrew the top of the vial and transfer the contents to a sterile centrifuge tube containing 9 mL of the recommended medium. Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10 minutes at 125 × g). Discard the supernatant, and resuspend the cells in 1 or 2 mL of complete growth medium. Transfer the cell suspension into the culture vessel containing the complete growth medium and mix thoroughly by gentle rocking.
5. Examine the cell cultures after 24 hours and subculture as needed.
(4)Processing Flask Cultures: Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells, incubated to ensure cell growth and then filled completely with medium for shipping. Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes unusual pH shifts (yellow or purple color from the phenol red), turbidity, or particles. With an inverted microscope at low power (100×) check the medium for evidence of microbial contamination as well as the morphology of the cells. See page 6 for more details on examining cell cultures. 烜雅生物发布 质量可靠,售后有保障 如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发 人肺小细胞肺癌_NCI-H2227相关产品: