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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 细胞类型:
科研
- 品系:
详情请咨询我司客服
- 组织来源:
详情请咨询我司客服
- 物种来源:
鼠/人/其它
- 细胞形态:
上皮样/成纤维样/其它
- 器官来源:
详情请咨询我司客服
- 运输方式:
常温运输/干冰运输
- 年限:
详情请咨询我司客服
- 生长状态:
贴壁/悬浮
- 英文名:
SUNE-1亚株13-9B
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:

(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人鼻咽癌细胞系_SUNE-1亚株13-9B相关产品:
| WISH(羊膜细胞) |
| H08910(卵巢癌细胞) |
| Bcap-37(乳腺癌细胞) |
| Hela(宫颈癌细胞) |
| PC-3(人前列腺癌细胞) |
| SGC7901(胃腺癌细胞) |
| 786-0(肾透明细胞腺癌) |
| 6T-CEM(T细胞白血病) |
| Jecket(淋巴瘤细胞) |
| JAR(胚绒毛癌细胞) |
| PC-12未分化(大鼠肾上腺嗜铬细胞瘤) |
| WI-38(人二倍体胚肺细胞) |
| THP-1(人单核细胞) |
| T-24(膀胱变移细胞癌) |
| A375(人类恶性黑色素瘤) |
| CHRF(人巨核细胞白血病) |
| K562(慢性髓原白血病) |
| RD(恶性胚胎横纹肌瘤) |
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文献和实验400元 BHK-21 [C-13] GNHa 1 仓鼠 幼仓鼠肾细胞 400元 CHL GNHa 2 仓鼠 肺细胞 400元 79 GNHa 6 仓鼠 肺细胞 400元 CHO GNHa 3 仓鼠 卵巢细胞 400元 CHO/dhFr- GNHa 4 仓鼠 卵巢细胞,二氢叶酸还原酶缺陷 1000元 CHO-K1 GNHa 7 仓鼠 卵巢细胞亚株 800元 Lec1 GNHa 8 仓鼠 卵巢细胞 1000元 R 1610
芯片。而基因芯片中,最成功的是DNA芯片,即将无数预先设计好的寡核苷酸或cDNA在芯片上做成点阵,与样品中同源核酸分子杂交的芯片。基因芯片的基本原理同芯片技术中杂交测序(sequencing by hybridization,SBH)。即任何线状的单链DNA或RNA序列均可被分解为一个序列固定、错落而重叠的寡核苷酸,又称亚序列(subsequence)。例如可把寡核苷酸序列TTAGCTCATATG分解成5个8nt亚序列: (1) CTCATATG (2) GCTCATAT (3) AGCTCATA
大鼠心肌细胞RSC96 大鼠雪旺细胞3、仓鼠类 BHK 幼仓鼠肾细胞 R 1610 中国仓鼠体细胞 CHL 中国仓鼠肺细胞 CHO 中国仓鼠卵巢细胞 V79 中国仓鼠肺细胞 CHO-K1 卵巢细胞亚株 CHO/dhFr- 中国仓鼠二氢叶酸还原酶缺陷细胞 Lec1 卵巢细胞 4、人类 HFL
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