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- 文献和实验
- 技术资料
- 库存:
100
- 细胞类型:
科研
- 品系:
详情请咨询我司客服
- 组织来源:
详情请咨询我司客服
- 物种来源:
鼠/人/其它
- 细胞形态:
上皮样/成纤维样/其它
- 器官来源:
详情请咨询我司客服
- 运输方式:
常温运输/干冰运输
- 年限:
详情请咨询我司客服
- 生长状态:
贴壁/悬浮
- 英文名:
CNE-2Z
产品简介:
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
生物安全:
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装:
提供新鲜或者冻存的细胞
使用方法:
如是新鲜细胞,客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时,再进行后续的实验操作;如是冻存细胞, 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。
细胞培养 :
(1)Getting Started with an ATCC Cell Line:
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.
(2)细胞图片:

(3)Initiating Frozen Cultures:
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking.
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠,售后有保障
如运输过程中导致细胞污染或者死亡,我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
人鼻咽癌母系细胞_CNE-2Z相关产品:
| LS 174T(结肠腺癌细胞) |
| SK-N-MC(神经上皮瘤细胞) |
| SK-N-MC(神经上皮瘤细胞) |
| BxPC-3(原位胰腺腺癌细胞) |
| BxPC-3(原位胰腺腺癌细胞) |
| KP-N-NS(肾上腺神经母细胞瘤细胞(脑转移)) |
| AsPC-1(转移胰腺腺癌细胞) |
| AsPC-1(转移胰腺腺癌细胞) |
| U-2 OS(骨肉瘤细胞) |
| U-2 OS(骨肉瘤细胞) |
| GBC-SD(胆囊癌细胞) |
| RD(恶性胚胎横纹肌瘤细胞) |
| RD(恶性胚胎横纹肌瘤细胞) |
| HCCC-9810(胆管细胞型肝癌细胞) |
| A-673(横纹肌瘤细胞) |
| SMMC-7721(肝癌细胞) |
| SMC-1(胸膜细胞瘤) |
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文献和实验人头颈部肿瘤AM 人腺样囊性癌细胞(高转移)A2 人腺样囊性癌细胞A83 人腺样囊性癌细胞Hep-2 人喉表皮癌细胞KB 人口腔上皮癌细胞CNE-2Z 人鼻咽癌母系细胞肺癌A549 人肺腺癌细胞801-D 人巨细胞性肺癌细胞肺793 肺腺癌细胞H125 人肺癌细胞NCI-H460 人肺癌细胞LTEP-Sm1 人小细胞肺癌细胞(SCLC)消化系统肿瘤 HT-29 人结肠癌细胞 PC-3 人胰腺癌细胞 SW480 人结肠癌细胞
日》,其中有一份材料指出从 2007 年至 2018 年的 11 年间,以李红良为第一或者通讯作者发表的八篇文章中存在十处错误。2018 年 3 月 10 日,「知识分子」公众号继续跟进,发文《十七年前,暨南大学硕士 SCI 论文高产神话的背后》,指出 2001 年李红良发表在 Cell Research 的名为《肝素对人鼻咽癌 CNE2 细胞凋亡的作用》与发表在 Acta Pharmacol Sin(《中国药理学报》)的名为《肝素和阿霉素对人鼻咽癌 CNE2 细胞增殖与凋亡的协同作用》存在一图多用
表皮癌 QGY-7701 肝癌 A673 横纹肌癌 QGY-7703 肝癌 Tca-8113 舌鳞癌 SMMC-7721 肝癌 Acc-2 涎腺腺样囊性癌 786-O 肾透明细胞腺癌 Acc-3 涎腺腺样囊性癌 PC-3 前列腺癌 KB 口腔表皮样癌 T-24 膀胱变移细胞癌 HEp-2 喉表皮样癌 SCaBER 膀胱鳞癌 CNE 鼻咽癌 Ho-8910 卵巢癌 TT 髓性甲状腺癌 L1 卵巢
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