Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Xenopuslaevis
适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Xenopuslaevis
免疫原:
详见说明书
亚型:
IGg
形态:
粉末、液体、冻干粉
应用范围:
IF=1:50-200
浓度:
1mg/ml
保存条件:
-20 °C
规格:
100ul
FITC标记的高尔基体外周膜蛋白p65抗体
英文名称
Anti-GRASP65/FITC
中文名称
FITC标记的高尔基体外周膜蛋白p65抗体
别 名
Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; Golgi reassembly and stacking protein 65 kDa; Golgi reassembly stacking protein 1 65kDa; Golgi reassembly stacking protein 1; Golgi reassembly stacking protein of 65 kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH 5; GOLPH5; Gorasp 1; Gorasp1; GORS1_HUMAN; GRASP 65; GRASP65; MGC118894; MGC118897; P65; FLJ23443.
规格价格
100ul/2980元 购买 大包装/询价
说 明 书
100ul
研究领域
细胞生物 免疫学 细胞凋亡 细胞周期蛋白 细胞自噬
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Xenopuslaevis
产品应用
IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
46kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human GRASP65
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
Subunit: Homodimer. Forms higher order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115. Interacts with TMED2 and TMED3 (By similarity).
Post-translational modifications: Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimmers into higher order oligomers, leading to cisternal unstacking. Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity).
Similarity: Belongs to the GORASP family. Contains 1 PDZ (DHR) domain.
Database links: UniProtKB/Swiss-Prot: Q9BQQ3.3
Entrez Gene: 64689 Human
Omim: 606867 Human
SwissProt: Q9BQQ3 Human
Unigene: 613614 Human
Unigene: 721946 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.