用于3D或更高级别的细胞培养的VitroGel 3D

用于3D或更高级别的细胞培养的VitroGel 3D
VitroGel® 3D High Concentration

 

VitroGel 3D高浓度


VitroGel®3D高浓度是一种可调的无异种（无动物来源）水凝胶系统，可最大限度地灵活操作3D细胞培养环境以满足不同需求。VitroGel 3D高浓度配有VitroGelDilution Solution，可将最终水凝胶强度从10 Pa调整到4000 Pa。水凝胶的可调性使研究人员能够为细胞生长创造优化的环境。VitroGel 3D高浓度水凝胶基质结构有利于细胞球体的形成、悬浮细胞或需要低细胞-基质相互作用的细胞。


VitroGel高浓度水凝胶是我们的无氙可调水凝胶，适用于希望完全控制细胞培养环境的生物物理和生物特性的研究人员。水凝胶的可调性使其能够为细胞生长创造优化的环境。水凝胶系统具有中性pH、透明、可渗透和可渗透性

Specifications

Contents	VitroGel® 3D High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL
Hydrogel Formulation	Xeno-free tunable hydrogel, pure and unmodified.
Use	Good for cell spheroid formation, suspension cells or cells require low cell-matrix interactions
Mix & Match	Can be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix.
Operation	Room temperature
Hydrogel Strength	10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations.
pH	Neutral
Color	Transparent
Cell Harvesting	VitroGel Organoid Recovery Solution 5-15 min cell recovery
Injectable	Injectable hydrogel
Storage	Store at 2-8°C. Ships at ambient temperature
Number of Uses	Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well

Figure 1. Beta Lox 5 (BL5) cells 3D culture in VitroGel 3D system.
A. BL5 cells culture on the surface of regular tissue culture treated well plate (control); B. Normal human islets grew in suspension culture (comparison); C. 3D culture of BL5 cells in VitroGel 3D at Day 1; D. 3D culture of BL5 cells in VitroGel 3D at Day 7. Under 3D culture of VitroGel 3D, BL5 cells form islet-like structures very similar to normal human islets. The hydrogel is prepared at 1:3 dilution. The images were taken at 10X magnification.

Figure 2. CD8+ T cells 3D culture in VitroGel 3D system.
CD8+ T cells culture grew in suspension culture (control); B. 3D culture of CD8+ T cells in VitroGel 3D at Day 7.  CD8+ T cells are vibrant in 3D culture conditions of VitroGel 3D. The cells can easily move within the unmodified hydrogel matrix. The hydrogel is prepared at 1:3 dilution. The images were taken at 10X magnification.

2D Coating Applications

Figure 3. Human colon cancer cells (HCT 116) cells cultured on top of VitroGel 3D hydrogel.
A thick hydrogel coating plate has been prepared by mixing VitroGel 3D with PBS at 1:1 ratio. A 300 µL mixture has been added to a well of a 24-well plate and stabilization at room temperature for 20 minutes before adding cells on top of the hydrogel. Cell spheroids form on the top of the hydrogel. Cells seeded at 2.5-10×10⁵ cells/mL.

Figure 4. Comparison of long-term neuronal culture seeded onto thick hydrogel mats.
Cells are stained with Beta-III-Tubulin (green) cytoskeleton marker and their nuclei are counter-stained with DAPI (blue). Cells spread out and form neural-like networks as early as day 3 post-differentiation, with comparable efficacy between VitroGel 3D and Matrigel, based on cell survival, culture spreading, and morphological analysis reached between days 7 and 9. On Matrigel mats, cell culture health and viability drop off sharply once day 9 has passed, with most cells detaching and neurites retracting by day 14 and the vast majority of cells gone by day 21. If grown onto VitroGel 3D mats, differentiated B35 neurons have a tendency to self-organize into 3D clusters very early on (Day 7), assuming a mixed 2D/3D cell culture for the first two weeks of the time course. By Day 21, these cells have migrated into self-assembled 3D clusters, embedded into the thick hydrogel matrix, with very few cells between the clusters, but without any significant cell death.

Figure 5. Human Lymphoblastoid Priess cells cultured on top of VitroGel 3D hydrogel.
A. Priess cells grown in suspension (control); B. Priess cells grown on top of VitroGel 3D at day 7. A hydrogel substance can be prepared with different stiffness by adjusting the dilution of VitroGel 3D from 1:1 to 1:3 ratio. Cells seeded on the top of the hydrogel form cell spheroids form on the top of the hydrogel. The hydrogel provides a soft substance for cells to attach and grow.