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- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
蛋白激酶B品牌
- 抗体英文名：
Anti-AKT1/PKB
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海博湖
- 库存：
大量
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
大量
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human PKB C-terminus
- 规格：
0.2ml/200μg
英文名称 Anti-AKT1/PKB
中文名称 蛋白激酶B品牌
别 名 AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK Alpha;
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep
产品类型 一抗
研究领域 肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 56kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PKB C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
蛋白激酶B品牌产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
Function : AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory
14-3-3
proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity.
Subunit : Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting
14-3-3
binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.
Subcellular Location : Cytoplasm. Nucleus. Cell membrane (By similarity). Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1) (By similarity). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Post-translational modifications : O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.
Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells. Ser-473 phosphorylation is enhanced by signaling through activated FLT3. Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase. The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase.
Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome. Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. Also ubiquitinated by TRIM13 leading to its proteasomal degradation.
Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition.
DISEASE : Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Genetic variations in AKT1 may play a role in susceptibility to ovarian cancer.
Defects in AKT1 are a cause of Proteus syndrome (PROTEUSS) [MIM:176920]. A highly variable, severe disorder of asymmetric and disproportionate overgrowth of body parts, connective tissue nevi, epidermal nevi, dysregulated adipose tissue, and vascular malformations. Many features of Proteus syndrome overlap with other overgrowth syndromes.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Database links :
Entrez Gene: 207 Human
Entrez Gene: 11651 Mouse
Entrez Gene: 24185 Rat
Omim: 164730 Human
SwissProt: O57513 Chicken
SwissProt: P31749 Human
SwissProt: P31750 Mouse
SwissProt: P47196 Rat
Unigene: 525622 Human
Unigene: 6645 Mouse
Unigene: 11422 Rat
激酶和磷酸酶（Kinases and Phosphatases）
Akt/PKB(Protein kinase B;Protein kinase beta, gamma;PKB gamma;STK-2 )是丝氨酸/苏氨酸蛋白激酶家族成员，可使蛋酸化，也可自身磷酸化,在调节细胞存活中起重要作用。
Akt1/PKBa是一种丝氨酸/苏氨酸蛋白激酶，其激酶活力在包括细胞凋亡、糖原合成和细胞生长等的各种细胞功能中起着关键作用。Akt1/PKBa 被各种生长因子和存活因子所活化。其308位苏氨酸残基、473位丝氨酸残基和474位酪氨酸残基的磷酸化对于该酶的充分活化是不可或缺的。Akt1/PKBa通过对包括胱冬酶-9在内的几种靶标的磷酸化和失活而抑制细胞凋亡。 Akt1/PKBa 通过对糖原合酶激酶-3a和3b的磷酸化和失活来调节糖原合成。已鉴定了3种亚型的哺乳动物Akt1/PKBa。在很多种癌症中AKT都有非正常表达或过表达。
Murine thymoma viral(v-akt) oncogene homolog-2(AKT-2;PRKBB)—蛋白激酶AKT-2，被认为也是原癌基因之一。受PDGF，EGF，FGF激活，经过phosphatidylinositol 3-kinase作用而活化。通过对凋亡调控蛋白的磷酸化而灭活其活性，抑制细胞的凋亡.
此抗体与Akt-1，Akt-2，Akt-3均有交叉反应。
蛋白激酶B品牌具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务，可根据您科研的需求，满足您的需要，质量保证，价格合理。
一、标记流程
委托标记服务，请下载并详细填写《委托标记申请单》，待我公司标记室技术人员研究、确认后答复，并办理委托标记事宜。
二、各种标记物制备的收费标准（RMB）
我们不但提供抗体和大分子蛋白的标记服务，还提供小分子多肽及小分子蛋白的各种标记服务，提供标记化合物的服务（此化合物必须适用于标记），价格另议。
一抗和二坑的区别：
第一抗体就是平常所说的抗体，即能和抗原特异性结合。
第二抗体是能和抗体结合的，即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体，二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说，抗原进入机体刺激机体免疫系统产生免疫应答，由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团，而且一抗可以至少结合两种其他基团（底物和二抗）。
一抗：可以特异结合底物，就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗：可以和一抗结合，并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），则不需要二抗。但这样成本很高，因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记，再来检测一抗。而一抗识别底物。这样，当一抗结合到底物上，就可以通过二抗检测出来。
Western Blot封闭液2（奶粉，pH7.5） 进口/国产
哺乳动物蛋白抽提试剂 进口/国产
过铵（APS） 进口/国产
0.5M EDTA (PH8.0) 进口/国产
Streptavidin-HRP（IHC工作液） 进口/国产
酰基辅酶A合成酶家族4抗体 Anti-ACSF4 WB IHC-P IHC-F IF 100ul
酸性酶样蛋白2抗体 Anti-ACPL2 WB IHC-P IHC-F IF 100ul
AHNAK核蛋白2抗体 Anti-AHNAK2 WB IHC-P IHC-F IF 100ul
二腺苷核糖基化因子6相互作用蛋白抗体 Anti-ARL6 WB IHC-P IHC-F IF 100ul
p53诱导蛋白5抗体 Anti-ANO9 WB IHC-P IHC-F IF 100ul
微管相关蛋白4抗体 Anti-MAP4 WB IHC-P IHC-F IF 100ul
26S蛋白酶调节亚型7抗体 Anti-MSS1 WB IHC-P IHC-F IF 100ul
蛋白酪酸酶非受体型4抗体 Anti-MEG1 WB IHC-P IHC-F IF 100ul
脑肠肽O酰基转移酶抗体 Anti-MBOAT4 WB IHC-P IHC-F IF 100ul
转录调控因子MRG15抗体 Anti-MRG15 WB IHC-P IHC-F IF 100ul
Anglne(人卵巢癌细胞) 5×106cells/瓶×2
CL-0149MCF-7(人癌细胞)5×106cells/瓶×2
ERBB4 Others Rat 大鼠 HER4 / ErbB4 人细胞裂解液 (阳性对照)
NIH/3T3细胞，鼠成纤维细胞系 人Butts淋巴瘤细胞系,CA46细胞 小鼠胚胎成纤维细胞;3T3-L1
猪肾细胞;LLC-PK1
CXCL12 Others Human 人 CXCL12 / SDF1b 人细胞裂解液 (阳性对照)
人肺泡上皮细胞HPAEpiC
HA Others H1N1 甲型流感 H1N1 (A/Texas/05/2009) HA 人细胞裂解液 (阳性对照)
KM9304 EBV-转化人淋巴细胞(彝族)
CM-H099人真皮淋巴上皮细胞完全培养基100mL
PC-12(高分化)，PC12，大鼠肾上腺髓质嗜铬瘤分化细胞株(高分化)
人癌细胞;MDA-MB-231 人视网膜色素上皮细胞完全培养基 100mL
蛋白激酶B品牌Anglne(人卵巢癌细胞) 5×106cells/瓶×2
CL-0149MCF-7(人癌细胞)5×106cells/瓶×2
ERBB4 Others Rat 大鼠 HER4 / ErbB4 人细胞裂解液 (阳性对照)
NIH/3T3细胞，鼠成纤维细胞系 人Butts淋巴瘤细胞系,CA46细胞 小鼠胚胎成纤维细胞;3T3-L1
猪肾细胞;LLC-PK1
CXCL12 Others Human 人 CXCL12 / SDF1b 人细胞裂解液 (阳性对照)
蛋白激酶B品牌是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品，产品品质卓越，价格实惠，多种规格供应，售后完善。
中文名称 蛋白激酶B品牌
别 名 AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK Alpha;
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep
产品类型 一抗
研究领域 肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 56kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PKB C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
蛋白激酶B品牌产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
Function : AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory
Subunit : Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting
Subcellular Location : Cytoplasm. Nucleus. Cell membrane (By similarity). Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1) (By similarity). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Post-translational modifications : O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.
Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells. Ser-473 phosphorylation is enhanced by signaling through activated FLT3. Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase. The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase.
Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome. Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. Also ubiquitinated by TRIM13 leading to its proteasomal degradation.
Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition.
DISEASE : Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Genetic variations in AKT1 may play a role in susceptibility to ovarian cancer.
Defects in AKT1 are a cause of Proteus syndrome (PROTEUSS) [MIM:176920]. A highly variable, severe disorder of asymmetric and disproportionate overgrowth of body parts, connective tissue nevi, epidermal nevi, dysregulated adipose tissue, and vascular malformations. Many features of Proteus syndrome overlap with other overgrowth syndromes.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Database links :
Entrez Gene: 207 Human
Entrez Gene: 11651 Mouse
Entrez Gene: 24185 Rat
Omim: 164730 Human
SwissProt: O57513 Chicken
SwissProt: P31749 Human
SwissProt: P31750 Mouse
SwissProt: P47196 Rat
Unigene: 525622 Human
Unigene: 6645 Mouse
Unigene: 11422 Rat
激酶和磷酸酶（Kinases and Phosphatases）
Akt/PKB(Protein kinase B;Protein kinase beta, gamma;PKB gamma;STK-2 )是丝氨酸/苏氨酸蛋白激酶家族成员，可使蛋酸化，也可自身磷酸化,在调节细胞存活中起重要作用。
Akt1/PKBa是一种丝氨酸/苏氨酸蛋白激酶，其激酶活力在包括细胞凋亡、糖原合成和细胞生长等的各种细胞功能中起着关键作用。Akt1/PKBa 被各种生长因子和存活因子所活化。其308位苏氨酸残基、473位丝氨酸残基和474位酪氨酸残基的磷酸化对于该酶的充分活化是不可或缺的。Akt1/PKBa通过对包括胱冬酶-9在内的几种靶标的磷酸化和失活而抑制细胞凋亡。 Akt1/PKBa 通过对糖原合酶激酶-3a和3b的磷酸化和失活来调节糖原合成。已鉴定了3种亚型的哺乳动物Akt1/PKBa。在很多种癌症中AKT都有非正常表达或过表达。
Murine thymoma viral(v-akt) oncogene homolog-2(AKT-2;PRKBB)—蛋白激酶AKT-2，被认为也是原癌基因之一。受PDGF，EGF，FGF激活，经过phosphatidylinositol 3-kinase作用而活化。通过对凋亡调控蛋白的磷酸化而灭活其活性，抑制细胞的凋亡.
此抗体与Akt-1，Akt-2，Akt-3均有交叉反应。
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一、标记流程
委托标记服务，请下载并详细填写《委托标记申请单》，待我公司标记室技术人员研究、确认后答复，并办理委托标记事宜。
二、各种标记物制备的收费标准（RMB）
我们不但提供抗体和大分子蛋白的标记服务，还提供小分子多肽及小分子蛋白的各种标记服务，提供标记化合物的服务（此化合物必须适用于标记），价格另议。
一抗和二坑的区别：
第一抗体就是平常所说的抗体，即能和抗原特异性结合。
第二抗体是能和抗体结合的，即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体，二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说，抗原进入机体刺激机体免疫系统产生免疫应答，由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团，而且一抗可以至少结合两种其他基团（底物和二抗）。
一抗：可以特异结合底物，就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗：可以和一抗结合，并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），则不需要二抗。但这样成本很高，因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记，再来检测一抗。而一抗识别底物。这样，当一抗结合到底物上，就可以通过二抗检测出来。
Western Blot封闭液2（奶粉，pH7.5） 进口/国产
哺乳动物蛋白抽提试剂 进口/国产
过铵（APS） 进口/国产
0.5M EDTA (PH8.0) 进口/国产
Streptavidin-HRP（IHC工作液） 进口/国产
酰基辅酶A合成酶家族4抗体 Anti-ACSF4 WB IHC-P IHC-F IF 100ul
酸性酶样蛋白2抗体 Anti-ACPL2 WB IHC-P IHC-F IF 100ul
AHNAK核蛋白2抗体 Anti-AHNAK2 WB IHC-P IHC-F IF 100ul
二腺苷核糖基化因子6相互作用蛋白抗体 Anti-ARL6 WB IHC-P IHC-F IF 100ul
p53诱导蛋白5抗体 Anti-ANO9 WB IHC-P IHC-F IF 100ul
微管相关蛋白4抗体 Anti-MAP4 WB IHC-P IHC-F IF 100ul
26S蛋白酶调节亚型7抗体 Anti-MSS1 WB IHC-P IHC-F IF 100ul
蛋白酪酸酶非受体型4抗体 Anti-MEG1 WB IHC-P IHC-F IF 100ul
脑肠肽O酰基转移酶抗体 Anti-MBOAT4 WB IHC-P IHC-F IF 100ul
转录调控因子MRG15抗体 Anti-MRG15 WB IHC-P IHC-F IF 100ul
Anglne(人卵巢癌细胞) 5×106cells/瓶×2
CL-0149MCF-7(人癌细胞)5×106cells/瓶×2
ERBB4 Others Rat 大鼠 HER4 / ErbB4 人细胞裂解液 (阳性对照)
NIH/3T3细胞，鼠成纤维细胞系 人Butts淋巴瘤细胞系,CA46细胞 小鼠胚胎成纤维细胞;3T3-L1
猪肾细胞;LLC-PK1
CXCL12 Others Human 人 CXCL12 / SDF1b 人细胞裂解液 (阳性对照)
人肺泡上皮细胞HPAEpiC
HA Others H1N1 甲型流感 H1N1 (A/Texas/05/2009) HA 人细胞裂解液 (阳性对照)
KM9304 EBV-转化人淋巴细胞(彝族)
CM-H099人真皮淋巴上皮细胞完全培养基100mL
PC-12(高分化)，PC12，大鼠肾上腺髓质嗜铬瘤分化细胞株(高分化)
人癌细胞;MDA-MB-231 人视网膜色素上皮细胞完全培养基 100mL
蛋白激酶B品牌Anglne(人卵巢癌细胞) 5×106cells/瓶×2
CL-0149MCF-7(人癌细胞)5×106cells/瓶×2
ERBB4 Others Rat 大鼠 HER4 / ErbB4 人细胞裂解液 (阳性对照)
NIH/3T3细胞，鼠成纤维细胞系 人Butts淋巴瘤细胞系,CA46细胞 小鼠胚胎成纤维细胞;3T3-L1
猪肾细胞;LLC-PK1
CXCL12 Others Human 人 CXCL12 / SDF1b 人细胞裂解液 (阳性对照)
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2 从线粒体外膜脱落，进入细胞浆中。在细胞浆中，HK2 结合 NF-κB 的抑制因子 IκBα。 重要的是，HK2 发挥了不依赖于其经典代谢功能的新功能，即作为一个蛋白激酶磷酸化 IκBα 的 Thr 291 位点。该磷酸化促进了蛋白酶 μ-calpain 与 IκBα 的结合并进一步降解 IκBα，进而使转录因子 NF-κB 入核，最终促进了 PD-L1 的表达并导致了肿瘤的免疫逃逸。作者还发现，使用己糖激酶的抑制剂与 PD-1 抗体联用治疗小鼠胶质瘤，可以显著提升 PD-1 抗体的治疗
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三叶虫 大家好，我最近准备做NF-κB信号通路中的P65，P50还有IKK，IκB。请问各位买哪个公司的抗体好，中衫的怎样？ 望各位多多指教。谢谢！！ 三叶虫 顶自己一下。。 siru1234 你好，建议你买大品牌的抗体，质量有保证，我以前就曾用过abcom和sigma 的抗体，挺好用的。我们公司专门做血清的，如果你有需要可以联系，QQ：1137173658，电话：021
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