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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- CAS号：
201608-14-2
- 供应商：
上海研卉生物科技有限公司
- 保存条件：
低温
- 英文名：
Ac-DEVD-AFC
- 规格：
5mg
Ac-DEVD-AFC *CAS 201608-14-2*
| CAS | Molecular weight | Excitation peak (nm) | Emission peak (nm) | Extinction coefficient (cm-1 M-1) | Quantum yield |
| 201608-14-2 | 729.61 | 376 | 480 | 17000 | 0.53 |
Example protocol
At a glance
Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.
It can be used within 12 months from the date of receipt.
Procedure
Following protocol only provides a guideline, and should be modified according to your specific needs.
General Solution Caspase Assays Using AMC, AFC, pNA, R110 and ProRed Substrates
- Prepare a 10 mM stock solution in DMSO.
- Prepare a 2X caspase substrate (50 µM) assay solution as the following: 50 µL substrate stock solution, 100 µL DTT (1M), 400 µL EDTA (100 mM), 10 mL Tris Buffer (20 mM), pH =7.4.
- Mix equal volume of the caspase standards or samples with 2X caspase substrate assay solution, and incubate the solutions at room temperature for at least 1 hour.
- Monitor the fluorescence using a fluorescence microplate reader, or absorbance using an absorbance microplate reader.
Cell Caspase Assays Using Cell-Permeable FMK Caspase Probes
- Prepare a 2-5 mM stock solution in DMSO.
- Treat cells as desired.
- Prepare a 2X permeable caspase substrate (20 µM) assay solution by diluting the DMSO stock solution (from Step 2.1) in Hanks with 20 mM Hepes buffer (HHBS).
- Mix equal volume of the treated cells with 2X caspase substrate assay solution (from Step 2.3), and incubate the cells in a 37°C, 5% CO2 incubator for at least1 hour.
- Wash the cells with HHBS for at least once.
- Monitor the fluorescence intensity by a flow cytometer, a fluorescence microscope or a fluorescence microplate reader.
Cell Caspase Assays Using Cell-Permeable FMK Caspase Probes (For #13470-13476 only)
- Prepare a 250X stock solution by adding 50 µL DMSO into the vial.
- Treat cells as desired.
- Add 250 X DMSO stock solution into the cell solution at a 1:250 ratio (such as 2 µL to 500 µL cells), and incubate the cells in a 37°C, 5% CO2 incubator for 1 hour.
- Wash the cells with HHBS for at least once.
- Monitor the fluorescence intensity by flow cytometer, fluorescence microscopy or fluorescent microplate reader.
Calculators
Common stock solution preparation| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 137.06 µL | 685.298 µL | 1.371 mL | 6.853 mL | 13.706 mL |
| 5 mM | 27.412 µL | 137.06 µL | 274.119 µL | 1.371 mL | 2.741 mL |
| 10 mM | 13.706 µL | 68.53 µL | 137.06 µL | 685.298 µL | 1.371 mL |
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / |
| = |
| x |
| = |
Spectrum
Specifications
| Name | Excitation peak (nm) | Emission peak (nm) | Extinction coefficient (cm-1 M-1) | Quantum yield |
| Ac-DEVD-AFC *CAS 201608-14-2* | 376 | 480 | 17000 | 0.53 |
| Ac-DEVD-AMC *CAS 169332-61-0* | 339 | 439 | - | - |
| Ac-DEVD-CHO *CAS 169332-60-9* | - | - | - | - |
| Ac-DEVD-pNA *CAS 189950-66-1* | - | - | - | - |
| (Ac-IETD)2-R110 | 499 | 521 | 80000 | - |
| Ac-IETD-AFC *CAS 211990-57-7* | 376 | 480 | 17000 | 0.53 |
| Ac-IETD-AMC | 339 | 439 | - | - |
| Ac-IETD-CHO *CAS 191338-86-0* | - | - | - | - |
| FAM-VAD-FMK | 492 | 516 | 83000 | - |
| FITC-C6-DEVD-FMK | 489 | 515 | 73000 | - |
| mFluor™ 450-VAD-FMK | 405 | 444 | 35000 | - |
| mFluor™ 510-VAD-FMK | 408 | 503 | 25000 | - |
| SRB-VAD-FMK [Sulforhodamine B-VAD-FMK] | 557 | 576 | - | - |
| TF3-DEVD-FMK | 545 | 570 | 85000 | - |
| TF4-VAD-FMK | 577 | 601 | 90000 | - |
| (Z-DEVD)2-R110 | 499 | 521 | 80000 | - |
| Z-DEVD-AFC | 376 | 480 | 17000 | 0.53 |
| Z-DEVD-AMC | 339 | 439 | - | - |
| Z-DEVD-pNA | - | - | - | - |
| Z-DEVD-ProRed™ 620 | 530 | 619 | - | - |
| Z-IETD-AFC *CAS 219138-02-0* | 376 | 480 | 17000 | 0.53 |
| Z-IETD-pNA *CAS 219138-21-3* | - | - | - | - |
| Z-IETD-ProRed™ 620 | 530 | 619 | - | - |
Images
 Figure 1. Chemical structure for Ac-DEVD-AFC *CAS 201608-14-2* |
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- 作者
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文献和实验-DEVD-AMC（caspase-3四肽荧光底物），37°C反应1h，荧光分光光度计（Polarstar）分析荧光强度（激发光波长380nm，发射光波长为430-460nm）。 结果：[图9] 3 流式细胞术分析 方法：收获细胞正常或凋亡细胞？PBS洗涤？加Ac-DEVD-AMC?37°C反应1h?UV流式细胞计分析caspase-3阳性细胞数和平均荧光强度。 结果：[图10]
或凋亡细胞?PBS洗涤?制备细胞裂解液?加Ac-DEVD-AMC（caspase-3四肽荧光底物）?37°C反应1h?荧光分光光度计（Polarstar）分析荧光强度（激发光波长380nm，发射光波长为430-460nm）。结果如图10: 3 流式细胞术分析 方法：收获细胞正常或凋亡细胞?PBS洗涤?加Ac-DEVD-AMC?37°C反应1h?UV流式细胞计分析caspase-3阳性细胞数和平均荧光强度。结果如图11：
【求助】请教Z-VAD, Ac—DEVD—CHO和Z-Asp-crnk
fenghaozi 请教Z-VAD, Ac—DEVD—CHO和Z-Asp-crnk的中文名称各是什么 另外他们之间有何关系 作为对Caspase-3的抑制剂来讲，选择哪种最好？标准是什么？ 请教高手回答，谢谢 ioz2008sina Caspase抑制剂Z-VAD-FMK(Caspase Inhibitor Z-VAD-FMK)是一种可以穿透细胞膜的泛Caspase抑制
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