- ¥900
- affinity
- T0057
- 2025年09月03日
- WB
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
V5 tag antibody
- 抗体英文名：
V5 tag antibody
- 应用范围：
WB
- 抗原来源：
其它
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 是否单克隆：
多克隆
- 规格：
50μl
名 称:
V5 tag 抗体
货 号:
T0057
来 源:
Mouse
应 用:
WB
反 应:
All
浓 度:
1mg/ml
Alternative Names:Expand▼
V5-Tag;Protein Rev;
Applications:
WB(1:2000~1:20000), ELISA(peptide) 1:20000-1:40000
Reactivity:
All
Source:
Mouse
Clonality:
Monoclonal
Clone Number:
AFB17768
Purification:
Protein G purification.
Specificity:
T0057 detects a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Mouse IgG1 in 10 mM PBS (pH 7.4), 0.1mg/ml BSA , 50% glycerol and 1% ProClin™ 300.Store at -20 °C.Stable for 12 months from date of receipt.
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文献和实验To engineer or manipulate antibody or Fv-based molecules, isolation of the V region of the antibodies is necessary. A number of strategies can be adopted for amplification; one such approach is to use subgroup-specific oligonucleotides
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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