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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 英文名:
Recombinant Human CD6 Fc Chimera Protein, CF (100 ug)
- 规格:
100ug
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文献和实验cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
, and analysis. A protocol is described for enhanced detection of low?affinity interactions by generating multivalent complexes using Fc?fusion bait proteins and protein A microbeads, along with a statistical method for hit scoring and identification
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
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