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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month.
- 英文名:
5-Chlorodeoxyuridine; CldU
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
50-90-8
- 规格:
10 mM * 1 mL/50 mg/100 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥660.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥600.0 |
| 规格: | 100 mg | 产品价格: | ¥960.0 |
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5-Chloro-2'-deoxyuridine
CAS No. : 50-90-8
MCE 国际站:5-Chloro-2'-deoxyuridine
产品活性:5-Chloro-2'-deoxyuridine,一个胸腺嘧啶的类似物,可用于研究次氯酸对 DNA 和 DNA 前体的破坏作用。
研究领域:Others
作用靶点:Others
In Vitro: When 5-Chloro-2’-deoxyuridine (ClDU) is placed into tissue culture medium, mammalian cells incorporate the analog into DNA. It is observed that 10 μM concentration of 5-Chloro-2’-deoxyuridine in the media does not alter cell division kinetics. Previously it has been shown that 5-Chloro-2’-deoxyuridine is metabolized and incorporated into DNA using antibodies that bind selectively to DNA containing halogenated bases. In the studies reported here, 5-Chloro-2’-deoxyuridine is more similar to BrdU in acting as a T analog. The toxicity of 5-Chloro-2’-deoxyuridine could in part be attributed to inhibition of thymidylate synthase.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
96-well RNA In Situ Hybridization Protocol
). To stop development of individual wells, remove staining solution and add 200ul PBT. 26. To stop entire plate, rinse plate 3X with PBT. 27. Add 200ul 70% gycerol to each well. 28. Embryos are ready to mount or dissect once they have settled
Cell cycle analysis of Escherichia coli cells
: The C+D period is estimated from the initiation age (ai ), the generation time (τ) and the number of generations spanned per cell cycle. Example: 4-origin-cells: 23 % Generation time (τ): 27 min Initiation age (ai ): 5 min
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