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PowerUp™ SYBR™ Green Master Mi

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  • 询价
  • ABI
  • A25778
  • 美国
  • 2025年12月24日
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    上海臻诺生物科技有限公司
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    • 库存：

      300

    • 供应商：

      上海臻诺生物科技有限公司

    • 保存条件：

      低温

    PowerUp SYBR Green 预混液

    适用于所有qPCR仪器的通用型预混液

    PowerUp™ SYBR™ Green 预混液的特性包括：

    • 双重热启动机制提供了出众的特异性
    • 在较宽的动态范围内都能获得可重复性的 Ct 值
    • 适用于标准或快速循环，<50分钟内即可获得结果
    • 采用 UN G和 dUT P配方，可防止残留 PCR 产物污染
    • 制备好qPCR反应板后，可以在室温下稳定保存72小时
    • 兼容多种仪器
    货号：  A25778
     相关应用： 实时定量 PCR

    https://img1.dxycdn.com/p/s14/2024/0419/596/2726211793721711871.jpg
    PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

    PowerUp™ SYBR® Green Master Mix features include:
    • A dual hot-start mechanism for excellent specificity
    • Highly reproducible CTs over a broad dynamic range
    • Inclusion of UDG to help prevent carryover contamination
    • Stability of pre-assembled reactions for up to 72 hours
    • Compatibility with most real-time qPCR instruments

    Exceptional specificity with dual hot-start mechanism
    Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

    Tight reproducibility in CTs over a wide dynamic range
    PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

    UDG included for carryover contamination control
    The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

    Stability of pre-assembled reactions
    The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios    1.

    Broad instrument compatibility
    PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.



     
    Detection Method: SYBR
    For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
    Reaction Speed: Fast or Standard
    PCR Method: qPCR
    Sample Type (General): DNA
    Number of Reactions: 5000 reactions
    Polymerase: Dual-Lock™ Taq DNA Polymerase
    Product Line: PowerUP™, SYBR®
    Product Size: 10 x 5 mL
    Shipping Condition: Wet Ice

    内容及储存

    The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 X 5 mL tube, sufficient for 5000 20-µL reactions (10 µL Master Mix per reaction).

    Store at 2–8°C.
    现货订购  顺丰包邮  咨询热线：18221746381

     

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    相关实验

    • SYBR Green Quantitative PCR Protocol

      12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F

    • REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL

      1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度，需要摸索，从200nM到700nm等，设置不同浓度。3、每个引物浓度，从well 1到12设置4个样本，阳性cDNA 1和2，NTC以及H2O，做triplicate。4、若用ABI7000或7700，所有的PCR条件可设置成一致的，减少麻烦，当然，引物

    • Vybrant® DyeCycle Green and Orange Stains

      Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO

    查看更多相关实验 >
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