组蛋白H3 K9甲基转移酶抗体0.2ml/200μg

组蛋白H3 K9甲基转移酶抗体0.2ml/200μg现货供应，货期短，为您提供售前、售中、售后服务，保证实验结果，无效可以免费退换，提供免费代测服务，产品效价高、种类全、价格实惠 ，欢迎来电咨询！
英文名称 Anti-KMT1A/SUV39H1
中文名称 组蛋白H3 K9甲基转移酶抗体0.2ml/200μg
别 名 H3 K9 HMTase; H3 K9 HMTase1; H3-K9-HMTase 1; Histone H3 K9 methylation; Histone H3 Lys 9 methylation; Histone H3-K9 methyltransferase 1; Histone H3-K9 methyltransferase1; Histone lysine N methyltransferase H3 lysine 9 specific 1; Histone lysine N methyltransferase, H3 lysine 9 specific 1; Histone-lysine N-methyltransferase SUV39H1; KMT1 A; Lysine N methyltransferase 1A; Lysine N-methyltransferase 1A; MG44; mIS6; Position-effect variegation 3-9 homolog; Su(var)3 9 homolog 1; Su(var)3-9 homolog 1; Suppressor of variegation 3 9 homolog 1 (Drosophila); Suppressor of variegation 3-9 homolog 1; SUV39 H1; SUV39H; SUV39H1; SUV91_HUMAN.

浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 染色质和核信号 细胞周期蛋白
蛋白分子量 predicted molecular weight：48kDa  
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Histone H3 Lys 9 methylation/SUV39H1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

组蛋白H3 K9甲基转移酶抗体0.2ml/200μg产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell：
upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.  
Function : Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.
Subunit ：
nteracts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, KIAA1967/DBC1, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Interacts (via SET domain) with MECOM; enhances MECOM transcriptional repression activity (By similarity).  
Subcellular Location ：
Nucleus. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin.  
组蛋白H3 K9甲基转移酶抗体0.2ml/200μg抗体的多样性：
抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。

组蛋白H3 K9甲基转移酶抗体0.2ml/200μg实验步骤
① 滴加0.01mol/L，pH7.4的PBS于待检标本片上，10min后弃去，使标本保持一定湿度。
② 滴加适当稀释的荧光标记的抗体溶液，使其完全覆盖标本，置于有盖搪瓷盒内，保温一定时间（参考：30min）。
③ 取出玻片，置玻片架上，先用0.01mol/L，pH7.4的PBS冲洗后，再按顺序过0.01mol/L，pH7.4的PBS三缸浸泡，每缸3-5 min，不时振荡。
④ 取出玻片，用滤纸吸去多余水分，但不使标本干燥，加一滴缓冲甘油，以盖玻片覆盖。
⑤ 立即用荧光显微镜观察。观察标本的特异性荧光强度，一般可用“+”表示：
（-）无荧光；（±）极弱的可疑荧光；（+）荧光较弱，但清楚可见；（++）荧光明亮；（+++ --++++）荧光闪亮。待检标本特异性荧光染色强度达“++”以上，而各种对照显示为（±）或（-），即可判定为阳性。
注意事项
1）对荧光标记的抗体的稀释，要保证抗体的蛋白有一定的浓度，一般稀释度不应超过1：20，抗体浓度过低，会导致产生的荧光过弱，影响结果的观察。
2）染色的温度和时间需要根据各种不同的标本及抗原而变化，染色时间可以从10 min到数小时，一般30 min已足够。染色温度多采用室温（25℃左右），高于37℃可加强染色效果，但对不耐热的抗原（如流行性乙型脑炎病毒）可采用0-2℃的低温，延长染色时间。低温染色较37℃30 min效果好的多。
3）为了保证荧光染色的正确性，首次试验时需设置下述对照，以排除某些非特异性荧光染色的干扰。
① 标本自发荧光对照：标本加1-2滴0.01mol/L，pH7.4的PBS。
② 特异性对照（抑制试验）：标本加未标记的特异性抗体，再加荧光标记的特异性抗体。
③ 阳性对照：已知的阳性标本加荧光标记的特异性抗体。
如果标本自发荧光对照和特异性对照呈无荧光或弱荧光，阳性对照和待检标本呈强荧光，则为特异性阳性染色。
4）一般标本在高压汞灯下照射超过3min，就有荧光减弱现象，经荧光染色的标本zui好在当天观察，随着时间的延长，荧光强度会

PDCD7  凋亡相关蛋白7抗体    特价促销
UBE1/E1 Ubiquitin Activating Enzyme  泛素激活酶E1抗体    特价促销
GAS2L1  生长停滞特异性蛋白2家族1抗体    特价促销
GAS2L3  生长停滞特异性蛋白2家族3抗体    特价促销
GilZ/TilZ  糖皮质激素诱导亮氨酸拉链蛋白抗体    特价促销
MIER2  中胚层诱导早期反应蛋白2抗体    特价促销
IEX1/Differentiation dependent gene 2 protein  分化相关基因2蛋白/立即早期反应蛋白抗体    特价促销
IL-4I1  白细胞介素4诱导蛋白1抗体    特价促销
JMJD6  凋亡细胞清除受体抗体    特价促销
MAGED1  黑色素瘤相关抗原D1抗体    特价促销
组蛋白H3 K9甲基转移酶抗体0.2ml/200μgELISA Kit for Human Keratinocyte growth factor    现货供应    15.6-1000 pg/mL
ELISA Kit for Human Keratinocyte proline-rich protein    现货供应    0.78-50 ng/mL
ELISA Kit for Human Ketohexokinase    现货供应    0.312-20 ng/mL
ELISA Kit for Human Kidney androgen-regulated protein    现货供应    0.156-10 ng/mL
ELISA Kit for Human Kin of IRRE-like protein 1    现货供应    0.312-20 ng/mL
ELISA Kit for Human Kinase suppressor of Ras 2    现货供应    78-5000 pg/mL
ELISA Kit for Human Kinesin heavy chain isoform 5A    现货供应    0.312-20 ng/mL
ELISA Kit for Human Kininogen-1    现货供应    1.56-100 ng/mL
ELISA Kit for Human KiSS-1 receptor    现货供应    1.56-100 ng/mL
ELISA Kit for Human Kit ligand    现货供应    31.2-2000 pg/mL
PDCD7  凋亡相关蛋白7抗体    特价促销
UBE1/E1 Ubiquitin Activating Enzyme  泛素激活酶E1抗体    特价促销
GAS2L1  生长停滞特异性蛋白2家族1抗体    特价促销
GAS2L3  生长停滞特异性蛋白2家族3抗体    特价促销
GilZ/TilZ  糖皮质激素诱导亮氨酸拉链蛋白抗体    特价促销
MIER2  中胚层诱导早期反应蛋白2抗体    特价促销
IEX1/Differentiation dependent gene 2 protein  分化相关基因2蛋白/立即早期反应蛋白抗体    特价促销
IL-4I1  白细胞介素4诱导蛋白1抗体    特价促销
JMJD6  凋亡细胞清除受体抗体    特价促销
MAGED1  黑色素瘤相关抗原D1抗体    特价促销
组蛋白H3 K9甲基转移酶抗体0.2ml/200μg具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。