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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
0.156 - 10 ng/ml
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F11029 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CTSD / Cathepsin D in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CTSD / Cathepsin D as low as 0.156 nanograms per millilter.About CTSD / Cathepsin D
CTSD / Cathepsin D is a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. P07339 NM_001909 NP_001900.1
CTSD ElisaKit, CPSD ElisaKit, CLN10 ElisaKit, Lysosomal aspartyl peptidase ElisaKit, Lysosomal aspartyl protease ElisaKit, ProCathepsin D ElisaKit, CatD ElisaKit, Cathepsin D ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CTSD / Cathepsin D
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.156
Precision
Intra-Assay: CV<4.9% / Inter-Assay: CV<8.5%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Wash Buffer (25x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
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