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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-9527R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-ALAS1/FITC
- 抗体名:
Anti-ALAS1/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat, Dog, Horse, Rabbit,
- 适应物种:
Human, Mouse, Rat, Dog, Horse, Rabbit,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-ALAS1/FITC |
| 中文名称 | Anti-ALAS1/FITC抗体 |
| 别 名 | mitochondrial; nonspecific; 5 aminolevulinate synthase; 5 aminolevulinate synthase nonspecific mitochondrial; 5 aminolevulinic acid synthase; 5-aminolevulinate synthase; 5-aminolevulinic acid synthase 1; Alas 1; ALAS 3; ALAS; ALAS H; ALAS N; ALAS-H; alaS1; ALAS3; ALASH; Aminolevulinate delta synthase 1; Aminolevulinic acid synthase 1; Delta ALA synthetase; Delta aminolevulinate synthase; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; HEM1_HUMAN; MIG 4; MIG4; Migration inducing protein 4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Horse, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 65kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALAS1/ALAS-H |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. Subunit: Homodimer. Subcellular Location: Mitochondrion matrix Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Database links: Entrez Gene: 211 Human Entrez Gene: 11655 Mouse Entrez Gene: 65155 Rat Omim: 125290 Human SwissProt: P13196 Human SwissProt: Q8VC19 Mouse SwissProt: P13195 Rat Unigene: 476308 Human Unigene: 290578 Mouse Unigene: 97126 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验对照。 二、注意事项 1、荧光染色后一般在 1h 内完成观察,或于 4℃ 保存 4h ,时间过长,可能会使荧光提前衰退。 2、每次试验均需设置以下三种对照: (1) 阳性对照:阳性血清+荧光标记物; (2)阴性对照:阴性血清+荧光标记物; (3)荧光标记物对照:PBS +荧光标记物。 三、免疫荧光双标的经验之谈 1、选取一抗时,要求来源于两种不同的动物,我用的是来源于家兔和大鼠的抗体,二抗则是不同荧光信号标记的,我用的是 donkey anti-rabbit-FITC(绿)和donkey anti
于细胞阳选,也能用于细胞阴选中。 为了评估 Strep-Tactin®TACS 琼脂糖柱细胞阴选的效率,实验从外周血单个核细胞(PBMC)样品中富集了 CD3- CD56+自然杀伤细胞(NK)。材料为Strep-Tactin®TACS 琼脂糖柱以及生物素化的抗体,仅 10 分钟,便完成了细胞阴选(图 1)。 流程如下: 阴选流程:生物素化抗体与 PBMC 孵育标记非目的细胞,加入至亲和层析柱后,即可收集目的细胞 实验方案 CD3- CD56+ NK 的细胞阴选 · 将 1 x
antibodies in 1% NGS/CMF-PBS. Make 4 mls anti-mouse FTIC diluted 1:150 Make 1.5 mls anti-rabbit FITC diluted 1:200 6. Bind secondary antibodies 45 minutes--use 300 ul/well. 7. Wash 3X with PBST, 5 minutes each wash-use ~1 ml/well. 8.
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