Phaseolus vulgaris Leucoagglut

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.

The unconjugated PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing

Unit Size
5 mg
Storage Instructions
2-8 °C
Sugar Specificity
Galactose, Complex Structures
Usage Summary
Reconstitution: The lectin should be reconstituted 10 mM phosphate, pH 8.0. If reconstituted in 1 ml of 10 mM phosphate, the resulting solution will be 5 mM phosphate, 10 mM NaCl with trace CaCl2. After reconstitution, and if appropriate for use, a preservative such as sodium azide (0.04%) may be added. If the lectin is to be used for mitogenic assays, filter solution through a sterile 0.22 µm filter immediately after reconstitution, aliquot and store frozen. NOTE: Mitogenic activity may be diminished by repeated freezing and thawing. Anterograde Neuronal Tracing: For neuronal transport studies, reconstitute in 0.2 ml of 10 mM sodium phosphate, pH 8.0.Both the pH and ionic strength of the reconstituting buffer are important for optimal anterograde transport. A specific protocol for this application is available on request.
Applications
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Conjugate
Unconjugated

欣博盛独家代理品牌vectorlabs，公司成立时，那时市面上还没有单克隆抗体、生物素-亲和素产品以及凝集素产品。1976, Vectorlabs率先纯化出凝集素产品，并迅速成为世界上该类产品的主要供应商。次年，Vetorlabs推出了生物素-亲和素系统。由于生物素和亲和素分子间极强的相互作用力，因此可以在生物素化的分子和结合有标记分子的亲和素间形成近似共价键的连接。Vectorlabs可提供抗原修复试剂、封闭试剂、复染试剂、亲和素/链霉亲和素-酶结合物、ABC免疫组化试剂盒、免疫组化显色底物、免疫组化抗体等。