Oil Red O is a fat-soluble diazo dye used for staining lipid and fat deposits in cells and tissues. While samples may be fresh, frozen or formalin fixed, Oil Red O is not compatible with paraffin embedded tissue sections. It is normal to observe Oil Red O precipitates. Therefore, a working solution must be prepared fresh through filtration using Whatman paper or a syringe-driven filter unit.
Kit Components
Cat. No.
# of vials
Name
Quantity
Storage
0843a
1
Oil Red O Stock
100 ml
room temperature
0843b
2
Fixative
100 ml
room temperature
Materials Supplied by User
Whatman Paper
Funnel
Deionized H2O (diH2O)
Phosphate Buffered Saline (PBS) - Cat. No. 0303
0.2µM syringe-driven filter unit (Millipore) – optional