Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
免疫原:
详见说明书
亚型:
IGg
形态:
粉末、液体、冻干粉
应用范围:
IF=1:50-200
浓度:
1mg/ml
保存条件:
-20 °C
规格:
100ul
FITC标记的环指蛋白20抗体
英文名称
Anti-RNF20/FITC
中文名称
FITC标记的环指蛋白20抗体
别 名
BRE1A; BRE1 A; E3 ubiquitin-protein ligase BRE1A; hBRE1; RING finger protein 20; BRE1A_HUMAN.
规格价格
100ul/2980元 购买 大包装/询价
说 明 书
100ul
研究领域
细胞生物 染色质和核信号 信号转导 表观遗传学
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用
IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
114kDa
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human RNF20
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: RNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes. It is recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Function: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Subunit: Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B.
Subcellular Location: Nuclear
Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity: Belongs to the BRE1 family. Contains 1 RING-type zinc finger.
Database links:
Entrez Gene: 56254 Human
Entrez Gene: 109331 Mouse
Omim: 607699 Human
SwissProt: Q5VTR2 Human
SwissProt: Q7TT11 Mouse
Unigene: 656088 Human
Unigene: 24765 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.