- $665
- LSBio
- 0.156 - 10 ng/ml
- LS-F21355
- 美国
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.156 - 10 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Performance 100% Guaranteed
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Product Description
LS-F21355 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CST3 / Cystatin C in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CST3 / Cystatin C as low as 0.094 nanograms per millilter.About CST3 / Cystatin C
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. P01034 NM_000099 NP_000090.1
CST3 ElisaKit, ARMD11 ElisaKit, BA218C14.4 (cystatin C) ElisaKit, Cystatin-3 ElisaKit, Cystatin 3 ElisaKit, Cystatin C ElisaKit, Cystatin-C ElisaKit, Gamma-trace ElisaKit, Post-gamma-globulin ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CST3 / Cystatin C
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant Mouse Cys-C.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.094 ng/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Biotinylated Detection Antibody (100x)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate (100x)
- HRP Conjugate Diluent
- Wash Buffer (25x)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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