Mouse/Human/Rat Phospho-CSF1R
/ CD115 / FMS (Tyr723) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1258- $740
- LSBio
- 详见官方网站
- LS-F1258
- 美国
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F1258 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human CSF1R / CD115 / FMS in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.About CSF1R / CD115 / FMS
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. P07333 NM_005211 NP_005202.2
CSF1R ElisaKit, CD115 antigen ElisaKit, CSF-1R ElisaKit, CSF-1-R ElisaKit, CSF-1 receptor ElisaKit, FMS ElisaKit, Fim-2 ElisaKit, FIM2 ElisaKit, FMS proto-oncogene ElisaKit, M-CSFR ElisaKit, HDLS ElisaKit, M-cfs receptor ElisaKit, C-FMS ElisaKit, CD115 ElisaKit, CSF1 receptor ElisaKit, CSFR ElisaKit, M-CSF-R ElisaKit, MCSF Receptor ElisaKit, Proto-oncogene c-Fms ElisaKit
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Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
CSF1R / CD115 / FMS
Reactivity
Mouse, Human, Rat
Specificity
CSFR (Phospho-Tyr723)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 15x Wash Buffer
- 100x Anti-CSFR (Phospho-Tyr723) Antibody (Rabbit Polyclonal)
- 100x Anti-CSFR Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based Phosphorylation ELISA Platform Overview
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spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
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