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- 详细信息
- 文献和实验
- 技术资料
- 库存:
490
- 英文名:
Recombinant Human Cytotoxic T-lymphocyte protein 4
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冻干蛋白置于-20℃以下可长期保存,室温
- 规格:
10μg|50μg|500μg|1mg
特别提示:包括重组人细胞毒性T淋巴细胞蛋白4(CTLA-4)(CD152)在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人细胞毒性T淋巴细胞蛋白4(CTLA-4)(CD152)
英文名称:Recombinant Human Cytotoxic T-lymphocyte protein 4
产品货号:重组人细胞毒性T淋巴细胞蛋白4(CTLA-4)(CD152)
产品规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Lys36-Asp161在C端含有His标签。
CTLA-4/CD152质量控制:>95%(还原性SDS-PAGE)
CTLA-4/CD152制剂:冻干品
CTLA-4/CD152保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
CTLA-4/CD152复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于CTLA-4/CD152:
Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
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文献和实验也能与B7―l/B7-2结合。已采用CTLA-4/Ig融合蛋白来封闭CD28与B7―1和B7-2结合提供的协同刺激信号,阻止T细胞活化,应用于免疫耐受的研究。
细胞形成以及浆细胞抗体的产生。 ②PD-1(CD279),与CTLA-4有24%同源性,胞质区有1个ITIM。PD-1在T细胞呈活化后诱导性表达,其配体为B7―H1/PD-L1(CD274)和B7-DC/PD-L2(CD273),P1)-I与相应配体结合后,胞质区ITIM中酪氨酸发生磷酸化,募集SHP-2,从而抑制T细胞的增殖,抑制IL-2、IL-10和IFN-7等细胞因子的产生,并对B细胞的增殖、分化与Ig分泌及Ig类别转换有抑制作用。 ③BTLA、CD272结构上与CTLA-4
Human CD4+CD25highCD127low/neg Regulatory T Cells
25, CD39/CD73, CD62L, CD45RO, CD127, glucocorticoid-induced tumor necrosis factor receptor (GITR), CTLA-4, and the forkhead/winged helix transcription factor (FOXP3), has been used to characterize Tregs. Tregs suppress T effector responses mainly
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