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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Uracil Excision Kit
- 供应商:
上海信裕生物科技有限公司
- 规格:
100 rxn
- 特异性切除尿嘧啶
- 形成单核苷酸缺口
- 适用于单链DNA和双链DNA

Uracil Excision Kit使用的Uracil Excision Enzyme可以特异性地切除尿嘧啶并形成一个单核苷酸缺口。Uracil Excision Enzyme包含有两种酶:尿嘧啶-DNA糖基化酶(UDG)和内切酶VIII(Endo VIII)。UDG催化从含尿嘧啶的DNA上释放尿嘧啶,产生一个脱碱基位点。Endo VIII会裂解这个脱碱基位点处的磷酸二酯键而形成一个单核苷酸缺口。本产品既可以切割单链DNA,也可以切割双链DNA。
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文献和实验Detection and Quantitation of Uracil DNA Glycosylase Activity
damage. One such repair mechanism is Base Excision Repair (BER). BER is a repair process initiated by a class of enzymes called glycosylases. These enzymes catalyze the hydrolysis of the N-glycosidic bond, thereby liberating the damaged or inappropriate
Analysis of Nuclear UracilDNA Glycosylase (nUDG) Turnover During the Cell Cycle
Uracil–DNA glycosylases (UDG/UNG) are enzymes that remove uracil from DNA and initiate base-excision repair. These enzymes play a key role in maintaining genomic integrity by reducing the mutagenic events caused by G:C to A:T transition
USER-Derived Cloning Methods and Their Primer Design
Uracil excision-based cloning through USER™ (Uracil-Specific Excision Reagent) is an efficient ligase-free cloning technique that comprises USER cloning, USER fusion, and USER cassette-free (UCF) USER fusion. These USER-derived cloning
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