Human CREBBP / CREB Binding Pr

Human CREBBP / CREB Binding Protein ELISA Kit (Sandwich ELISA) - LS-F36080

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LS-F36080-1
1 plate
$990 USD
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Popular CREBBP / CREB Binding Protein Elisa Kits

Rat CREBBP / CREB Binding Protein ELISA Kit (Sandwich ELISA) - LS-F36083
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Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

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Human CREBBP / CREB Binding Protein ELISA Kit (Sandwich ELISA) - LS-F7702
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Product Description

LS-F36080 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CREBBP / CREB Binding Protein in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CREBBP / CREB Binding Protein as low as 0.188 nanograms per millilter.
About CREBBP / CREB Binding Protein
CREBBP / CREB Binding Protein is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. Uniprot: Q92793 NCBI: NM_004380 NP_004371.2

Synonyms: CREBBP ElisaKit, CREB binding protein ElisaKit, CREB-binding protein ElisaKit, KAT3A ElisaKit, RSTS ElisaKit, Rubinstein-Taybi syndrome ElisaKit, RTS ElisaKit, CBP ElisaKit

More CREBBP / CREB Binding Protein Products
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CREBBP / CREB Binding Protein
Reactivity
 
Human
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.313 - 20 ng/ml
Sensitivity
 
0.188 ng/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Adhesive Plate Sealers
• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody (100x)
• HRP-Streptavidin Conjugate (100x)
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CREB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CREB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CREB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CREB can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
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