- $265
- Ybscience
- Yb--2510
- 美国/德国/中国
- 2025年07月16日
- WB IHC IF IP
- Human Mouse RatOtHumaneRat (Wide Ratange)
- 详见说明书
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- 文献和实验
- 技术资料
- 抗体名：
Histone H3K27me2 Antibody
- 抗体英文名：
Histone H3K27me2 Antibody
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB IHC IF IP
- 宿主：
Human Mouse RatOtHumaneRat (Wide Ratange)
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
2510
- 抗原来源：
Human Mouse RatOtHumaneRat (Wide Ratange)
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--2510
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 15kDa
- UniProt : Q16695
-
- Isotype : Rabbit IgG
- Clonality : Polyclonal
-
- Price : $265/100μg
- Availability : Ship next day
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;
Application:
WB IHC IF IP
Reactivity:
Human Mouse RatOtHumaneRat (Wide Ratange)
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 IP 1:50-1:200
 |
Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K27me2antibody|Dot-blot analysis of all sorts of methylation peptides using H3K27me2 antibody|Immunofluorescence analysis of 293T cell using H3K27me2 antibody. |
Purification:
Affinity-chromatography
Specificity:
Histone H3K27me2 Antibody detects endogenous levels of total Histone H3K27me2
Immunogen:
A synthesized peptide derived from human Histone H3K27me2
Description:
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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