- $615
- LSBio
- 详见官方网站
- LS-F1887
- 美国
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F1887 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human CLDN10 / Claudin 10 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.About CLDN10 / Claudin 10
CLDN10 / Claudin 10 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma.P78369 NM_006984 NP_008915.1
CLDN10 ElisaKit, CPETRL3 ElisaKit, Claudin 10 ElisaKit, OSP-L ElisaKit, OSP-like protein ElisaKit, Claudin-10 ElisaKit
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Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
CLDN10 / Claudin 10
Reactivity
Mouse, Human
Specificity
Claudin 10
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-Claudin 10 Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based ELISA Platform Overview
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
. 7. Wash 3X with DFH and 2X with HBSS. 8. Add 50 ml/well working substrate solution 0.5 ml 4.0% OPD 50 ml 30% H2O2 1.0 ml 10X Substrate buffer 8.5 ml di-H2O. 9. Incubate for 20 minutes at room temperature. 10. Add 25 ml/well 4.5N Sulfuric Acid
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