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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
62.5 - 4000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
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Popular CHI3L1 / YKL-40 Elisa Kits
Mouse CHI3L1 / YKL-40 ELISA Kit (Sandwich ELISA) - LS-F2800
Type: Quantitative Sandwich ELISA
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Reactivity: Mouse
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Product Description
LS-F23761 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CHI3L1 / YKL-40 in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CHI3L1 / YKL-40 as low as 37.5 picograms per milliliter.About CHI3L1 / YKL-40
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. P36222 NM_001276 NP_001267.2
CHI3L1 ElisaKit, 39 kDa synovial protein ElisaKit, ASRT7 ElisaKit, Cartilage glycoprotein 39 ElisaKit, CGP-39 ElisaKit, Chitinase-3-like protein 1 ElisaKit, gp39 ElisaKit, HCGP-39 ElisaKit, HCGP-3P ElisaKit, GP-39 ElisaKit, HC-gp39 ElisaKit, YKL40 ElisaKit, YYL-40 ElisaKit, YKL-40 ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CHI3L1 / YKL-40
Reactivity
Human
Specificity
This kit recognizes natural and recombinant Human GP39.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
37.5 pg/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Biotinylated Detection Antibody (100x)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate (100x)
- HRP Conjugate Diluent
- Wash Buffer (25x)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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