Human Phospho-CDKN1B / p27 Kip
1 (Thr198) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1367- $740
- LSBio
- 详见官方网站
- LS-F1367
- 美国
- 2025年07月09日
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- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F1367 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CDKN1B / p27 Kip1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.About CDKN1B / p27 Kip1
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. P46527 NM_004064 NP_004055.1
CDKN1B ElisaKit, KIP1 ElisaKit, MEN1B ElisaKit, P27 Kip1 ElisaKit, p27KIP1 ElisaKit, MEN4 ElisaKit, CDKN4 ElisaKit
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Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
CDKN1B / p27 Kip1
Reactivity
Human
Specificity
p27 Kip1 (Phospho-Thr198)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-p27 Kip1 (Phospho-Thr198) Antibody (Rabbit Polyclonal)
- 100x Anti-p27 Kip1 Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based Phosphorylation ELISA Platform Overview
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
我在Cell Signaling Technology上，查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP 9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641
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