Human CDKN1A / WAF1 / p21 ELIS

Human CDKN1A / WAF1 / p21 ELISA Kit (Sandwich ELISA) - LS-F51650

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LS-F51650-1
1 plate
$1,090 USD
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Product Description

LS-F51650 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CDKN1A / WAF1 / p21 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CDKN1A / WAF1 / p21 as low as 7.81 picograms per milliliter.
About CDKN1A / WAF1 / p21
CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. Uniprot: P38936 NCBI: NM_078467 NP_000380.1

Synonyms: CDKN1A ElisaKit, CDKN1 ElisaKit, CIP1 ElisaKit, CDK-interacting protein 1 ElisaKit, DNA synthesis inhibitor ElisaKit, p21CIP1 ElisaKit, MDA6 ElisaKit, PIC1 ElisaKit, SDI1 ElisaKit, WAF1 ElisaKit, CAP20 ElisaKit, CDK-interaction protein 1 ElisaKit, MDA-6 ElisaKit, p21 ElisaKit

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CDKN1A / WAF1 / p21
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human CDKN1A. No significant cross-reactivity or interference between human CDKN1A and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human CDKN1A and all the analogues, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
31.25 - 2000 pg/ml
Sensitivity
 
7.81 pg/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
 
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Adhesive Plate Sealers
• Sample Diluent
• Wash Buffer (25x)
• TMB Substrate
• Stop Solution
• Standard (Lyophilized)
• HRP Conjugate Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate (100x)
• Biotinylated Detection Antibody (100x)

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
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