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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Mouse, Human, Rat
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
详见官方网站
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F2202 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CDKN1A / WAF1 / p21 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.About CDKN1A / WAF1 / p21
CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P38936 NM_078467 NP_000380.1
CDKN1A ElisaKit, CDKN1 ElisaKit, CIP1 ElisaKit, CDK-interacting protein 1 ElisaKit, DNA synthesis inhibitor ElisaKit, p21CIP1 ElisaKit, MDA6 ElisaKit, PIC1 ElisaKit, SDI1 ElisaKit, WAF1 ElisaKit, CAP20 ElisaKit, CDK-interaction protein 1 ElisaKit, MDA-6 ElisaKit, p21 ElisaKit
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Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
CDKN1A / WAF1 / p21
Reactivity
Mouse, Human, Rat
Specificity
p21 Cip1
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-P21 Cip1 Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based ELISA Platform Overview
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All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
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