Mouse/Human/Rat Phospho-CDK1 /
CDC2 (Tyr15) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1469- $740
- LSBio
- 详见官方网站
- LS-F1469
- 美国
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F1469 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CDK1 / CDC2 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.About CDK1 / CDC2
Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. P06493 NM_001786 NP_001777.1
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Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
CDK1 / CDC2
Reactivity
Mouse, Human, Rat
Specificity
CDC2 (Phospho-Tyr15)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-CDC2 (Phospho-Tyr15) Antibody (Rabbit Polyclonal)
- 100x Anti-CDC2 Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based Phosphorylation ELISA Platform Overview
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
and stimulated with 0, 0.1, 1 and 10 µg/ml anisomycin for 15 minutes (Lanes 1–4). A Western blot was performed with 8 mg of extracts using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa assays were performed directly in the 96-well plates
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
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