- $665
- LSBio
- 62.5 - 4000 pg/ml
- LS-F2615
- 美国
- 2025年06月17日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
62.5 - 4000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F2615 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CDH3 / P-Cadherin in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CDH3 / P-Cadherin as low as 10 picograms per milliliter.About CDH3 / P-Cadherin
CDH3 / P-Cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. P22223 NM_001793 NP_001784.2
CDH3 ElisaKit, Cadherin-3 ElisaKit, HJMD ElisaKit, P-cadherin ElisaKit, CDHP ElisaKit, PCAD ElisaKit, Placental cadherin ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CDH3 / P-Cadherin
Reactivity
Mouse
Specificity
This kit is for the detection of oligomeric forms of natural and recombinant mouse P-Cadherin. No significant cross-reactivity or interference between P-Cadherin and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<6.8% Inter-Assay: CV%<7.8%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Biotinylated Detection Antibody (100x)
- ABC Complex (100x)
- Sample Diluent
- Detection Antibody Diluent
- ABC Complex Diluent
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
ELISA PROTOCOL 【Yale University 】
8.0Protocol:1) Add 50 µl of antigen to each well of the ELISA plate. I use a 1µg/ml of purified Sec4p most antigens will work in the 1-10 µg/mlrange, but you need to determine this emperically. Allow theantigen
ELISA PROTOCOL（Yale University）
AlkalinePhosphatase. I use a 1:500 dilution of rabbit anti-mouse IgG(Zymed)。 Incubate at room temperature for 1 hour. About 15 minbefore the end of the incubation start dissolving the p-nitrophenylphosphate substrate tablets. For each ELISA plate dissolve 1 tabletin
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