LS-F51641 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CD82 in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CD82 as low as 0.039 nanograms per millilter.
About CD82
This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. P27701NM_002231NP_002222.1
This assay has high sensitivity and excellent specificity for detection of human CD82. No significant cross-reactivity or interference between human CD82 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human CD82 and all the analogues, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.039 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components