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文献和实验A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
Drosophila Media Recipes and Methods
Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Cornmeal-molasses-yeast medium Cornmeal-sucrose-yeast medium Sucrose-yeast medium Cornmeal-dextrose-yeast medium
In-vitro Phagocytosis Assay of Macrophages
, set the pH to 5.6 and make volume to 1000 mL, and distribut in tubes or flasks. Autoclave the medium at 121ºC and 15 psi for 15 min. Add agar at the rate of 15 g/ L which will be resulted in a solid culture medium potato dextrose agar
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