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文献和实验to the animal and provides biologically meaningful samples. Only personnel with appropriate AHR training may handle live animals. Isoflourane inhalation: This should be given in sufficient dose to cause complete loss of breathing and pulse in the mouse. Apply
2+ endopeptidases, two Zn2+ -leucine aminopeptidases, and one Zn2+ carboxypeptidase (4 ,5 ). Pronase therefore has very broad specificity, and is used in cases where extensive or complete degradation of protein is required. It has been used, for example, to reveal
said that they ha ve measured elongation rates of 75 bp/second at 70°C, 24 bp/sec at 55°C, and 1.5 bp/sec at 35°C. Thus, extension occurs throughout the annealing step, further stabilizing the primer-template interactions, but increasing nonspecific
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