- $665
- LSBio
- 31.2 - 2000 pg/ml
- LS-F2969
- 美国
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
31.2 - 2000 pg/ml
- 供应商：
康朗生物
- 规格：
96T
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Popular CD27L / CD70 Elisa Kits
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Product Description
LS-F2969 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CD27L / CD70 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CD27L / CD70 as low as 10 picograms per milliliter.About CD27L / CD70
CD27L / CD70 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.P32970 NM_001252 NP_001243.1
CD70 ElisaKit, CD27LG ElisaKit, Ki-24 antigen ElisaKit, Surface antigen CD70 ElisaKit, CD27 ligand ElisaKit, CD27-L ElisaKit, CD70 antigen ElisaKit, CD70 molecule ElisaKit, TNFSF7 ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CD27L / CD70
Reactivity
Mouse
Specificity
This kit is for the detection of natural and recombinant mouse CD70. No significant cross-reactivity or interference between CD70 and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
31.2 - 2000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<5.1% Inter-Assay: CV%<6.2%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Biotinylated Detection Antibody (100x)
- ABC Complex (100x)
- Sample Diluent
- Detection Antibody Diluent
- ABC Complex Diluent
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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