- ¥3300
- IBL
- RE52651
- 2025年11月07日
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- 详细信息
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- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 规格：
96T
人脱氢表雄酮(DHEA)ELISA Kit详细说明
产品中文名称：人脱氢表雄酮(DHEA)ELISA Kit产品英文名称：Human Dehydroepiandrosterone,DHEA ELISA Kit
实验方法：酶联免疫法/酶免法（ELISA）
Catalog No. RE52651
| Kit size | 12 x 8 |
|---|---|
| Method | ELISA |
| Incubation time | 1 x 2 h, 1 x 30 min |
| Standard range | 20 - 2160 pg/ml |
| Specimen / Volumes | 50 µL saliva |
| Substrate / isotope | TMB 450 nm |
| Regulatory Status: | EU: CE |
The new IBL International DHEA Saliva ELISA correlates very well to the LC-MS/MS reference method, and is the only commercially available assay with clear specified reference values.
The normal DHEA concentration alters significantly with age, gender and time of the day. During the day, DHEA values have the highest level in the morning and decrease over the day to the lowest level during night time. This pattern is somewhat similar to Cortisol. For this reason clear indication about the reference values is of utmost importance.
Advantages of the improved IBL DHEA Saliva ELISA
- Calibration to the LC-MS/MS reference method
y = 0.91x – 13.35; r = 0.96; N = 130 - Measuring range up to 2,160 pg/mL
- Clearly defined reference values
- The TMB, stop solution and wash solution are interchangeable within Cortisol, Testosterone and Progesterone saliva ELISA (RE52611, RE52631 and RE52281)
The new reference ranges have been carefully determined on the basis of 140 day profiles, with respect to the gender, age and time of the day. Starting with batch no. EDE113, the following reference values have to be considered:
| Morning | Evening | |||||
| Age | n | 5%-95% percentile | Median | 5%-95% percentile | Median | |
| [pg/mL] | [pg/mL] | [pg/mL] | [pg/mL] | |||
|
Females
|
14-39 | 39 | 101.0-776.8 | 277.1 | 28.1-414.5 | 129.7 |
| 40-85 | 31 | 53.2-418.3 | 240.9 | 35.4-241.2 | 91.7 | |
| 14-85 (total) | 70 | 68.0-676.0 | 251.9 | 35.4-396.8 | 110.3 | |
|
Males
|
16-39 | 45 | 104.5-790.1 | 362.9 | 74.2-487.9 | 161.7 |
| 40-76 | 25 | 38.1-501.6 | 264.0 | 24.7-622.1 | 107.0 | |
| 16-76 (total) | 70 | 59.2-745.1 | 326.5 | 51.1-482.0 | 141.4 | |
It is recommended that each laboratory establishes its own range of normal values.
Numerous Application fields
Due to the ubiquitous occurrence of DHEA, its determination is useful in many fields of medical diagnostics and in research, not only in endocrinology and anti-aging medicine but also in neuroendocrinology and veterinary medicine. DHEA is a stress marker in psychology and in sport medicine.
Pulsatile dynamics
Due to the pulsatile dynamics of DHEA secretion repeated saliva sampling is recommended. We recommend collecting 3 to 5 saliva samples within 2 hours. In the laboratory, equal volumes of the individual saliva samples can be mixed. This mixed sample results in a mean free DHEA value, which represents the active hormone concentration in a reproducible way.
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文献和实验人脱氢表雄酮硫酸酯(DHEA-S)酶联免疫分析（ELISA）
人脱氢表雄酮硫酸酯(DHEA-S) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中脱氢表雄酮硫酸酯(DHEA-S) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人 脱氢表雄酮硫酸酯 (DHEA-S) 水平。用纯化的 人 脱氢表雄酮硫酸酯 (DHEA-S) 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔
C19 H28 O2 ， 3β -羟基 -5-雄烯 -17-酮。亦称脱氢异雄酮，缩写 DHA或 DHEA。是睾酮生物合成中的一个中间产物，主要是由 17β -羟甾醇脱氢酶而生成雄烯 -3β、 17β -二醇，△ 5 -3β -羟甾脱氢酶和△ 5 ，△ 4 -异化酶的作用生成睾酮。具有较弱的雄性激素作用，可以从尿中检测出来，经常以硫酸酯形态排出。认为这些是来自副肾的雄激素。在胎儿的副肾中产生的这种甾体，在肝等中接受 16α羟基化后，在胎盘中成为雌三醇
雌激素（雌酮、雌二醇、雌三醇），黄体酮、睾丸激素，DHEA-S（脱氢表雄酮）和可的松通常通过唾液检测。为什么是唾液呢？唾液检测是一种方便、便宜，最重要的是检测类固醇激素最准确的方法。科学研究表明血液中95-99%类固醇激素与载体蛋白相结合，这种形态的激素是不作用于靶器官的，只有未结合的游离片段才能分配到包括唾液腺的器官内，因此唾液激素水平代表了当前进入到靶器官具有生物活性的激素量，这类激素即是总血清激素中释放到脑、子宫、皮肤和乳腺等靶器官中的游离片段。由于这个原因，唾液激素水平通常与激素
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