基质胶 高浓度

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Source Engelbreth-Holm-Swarm Mouse Tumor Quality • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing • Extensive PCR testing is performed to screen for a number of pathogens • Tested and found negative for bacteria, fungi, and mycoplasma • Protein concentrations determined by Lowry method • Endotoxin units measured by Limulus Amoebocyte Lysate assay • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C • Biological activity is determined for each lot using a neurite outgrowth assay. Preparation and Storage Store at -20°C. Avoid multiple freeze-thaws. Application • Cell Growth and Differentiation • Metabolism/Toxicology Studies • Invasion Assays • in vitro and in vivo Angiogenesis Assays • in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice