Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing• Extensive PCR testing performed to screen for a number of pathogens• Tested and found negative for bacteria, fungi, and mycoplasma• Protein concentrations determined by Lowry method• Endotoxin units measured by Limulus Amoebocyte Lysate assay• Gel stability tested for a period of 14 days at 37°C• Biological activity determined for each lot using neurite outgrowth assay.SourceMEHS mouse tumorApplication• Cell Growth and Differentiation• Metabolism/Toxicology Studies• Invasion Assays• in vitro and in vivo Angiogenesis Assays• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice