Western blot detection of FADD in SW480, Jurkat and Hela cell lysates using FADD mouse mAb(dilution 1:500).Predicted band size:28kDa.Observed band size:28kDa.
Purification:
Affinity-chromatography
Specificity:
FADD Mouse mAb detects endogenous levels of total FADD
Immunogen:
Recombinant human FADD protein.
Description:
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors.The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation.Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.