Western blot detection of eIF2α in K562,COS7,3T3, C2C12,MCF-7,C6 and Hela cell lysates using eIF2α mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-eIF2α mouse mAb (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using eIF2α.
Purification:
Affinity-chromatography
Specificity:
eIF2α Mouse mAb detects endogenous levels of total eIF2α
Immunogen:
Purified recombinant human eIF2α protein fragments expressed in E.coli.
Description:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.