- $265
- Ybscience
- Yb--3324
- 美国/德国/中国
- 2025年07月15日
- WB ICC/IF
- Mouse
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Histone H2A.X(Phospho-Ser139) Mouse mAb
- 抗体英文名：
Histone H2A.X(Phospho-Ser139) Mouse mAb
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB ICC/IF
- 宿主：
Mouse
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
3324
- 抗原来源：
Mouse
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3324
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 15KDa
- UniProt : P16104
-
- Isotype : Mouse IgG1
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
H2A histone family, member X;H2A.X;H2a/x;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Histone H2A.X;Histone H2AX
Application:
WB ICC/IF
Reactivity:
Human,Mouse
Dilution:
WB 1:500-2000 ICC/IF 1:400
 |
Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa. |
 |
Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400). |
Purification:
Affinity-chromatography
Specificity:
Histone H2A.X(Phospho-Ser139) Mouse mAb detects endogenous levels of total Histone H2A.X(Phospho-Ser139)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Description:
Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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