WWestern blot detection of FEN-1 in Hela,Hela NE,Jurkat,K562 and 3T3 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Immunoprecipitation analysis of Hela cell lysates using FEN-1 mouse mAb.
Purification:
Affinity-chromatography
Specificity:
FEN-1 Mouse mAb detects endogenous levels of total FEN-1
Immunogen:
Purified recombinant human FEN-1 protein fragments expressed in E.coli.
Description:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.